Herein, the authors report an imaging technique to visualize nascent proteins by harnessing the emerging stimulated Raman scattering (SRS) microscopy coupled with metabolic incorporation of deuterium-labeled amino acids. Octadeuterated all-trans-retinyl acetate dose of 1.5 mg was given to a volunteer and blood samples were collected over a 32-day period. Spencer, K.A. J. Biophotonics, C.W. In recent years, an alternative tool for tagging biomols. SRS images from both live liver tissues (Figure, The ability to probe complex proteome metabolism with high sensitivity, resolution, and biocompatibility will help us to gain deep insight into protein metabolic regulation in biological systems under healthy and diseased conditions. biol. Saar, Brian G.; Freudiger, Christian W.; Reichman, Jay; Stanley, C. Michael; Holtom, Gary R.; Xie, X. Sunney. Xie, Vibrational spectroscopic imaging of living systems: an emerging platform for biology and medicine. Additionally, in contrast to coherent anti-Stokes Raman scattering (CARS), which is another coherent Raman technique, SRS signal is identical to spontaneous Raman spectra, linearly dependent on concentration, and free from non-resonant background. Anal. Berg, S. Pommert, R. Naskrecki, J.C. Mialocq, Cross phase modulation artifact in liquid phase transient absorption spectroscopy. Here we describe a method, termed SILAC, for stable isotope labeling by amino acids in cell culture, for the in vivo incorporation of specific amino acids into all mammalian proteins. Freudiger et al., Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Lys 63- and Lys 11-linked polyubiquitin chains, which are not typically assocd. azide, alkyne, or alkene groups have been esp. of water in which the D2O had been mixed was computed by the equation V2 = C1 V1/C2, where C1 and V1 are, resp., the concn. Appl. as they function within their native habitat. https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3cXktVGjtrc%253D&md5=661dcacd0279650a0e9364fde06f2f1f, In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons, https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3cXntlSjsbY%253D&md5=328f76d7d42e485603ac7d818b611eb7, Teaching old NCATs new tricks: using non-canonical amino acid tagging to study neuronal plasticity, https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3sXht1Gjs7vL&md5=0fd99be89250b08a93cbb20d8ae4a080, Zebrafish: an emerging model system for human disease and drug discovery, Clinical Pharmacology & Therapeutics (New York, NY, United States), https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD2sXotVGisr8%253D&md5=11a000b6faa063fb426a559400d4a8ca, Noncanonical Amino Acid Labeling in Vivo to Visualize and Affinity Purify Newly Synthesized Proteins in Larval Zebrafish, https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3MXhsVWltb3P&md5=538bea6b8716a9e8e7c02b27584096f4, Video-rate molecular imaging in vivo with stimulated Raman scattering, Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering, https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3cXhsVyrsbnO&md5=309bb071bb7559d66732ab2e2e52175c.
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